| # | Rider | Team | Age |
|---|---|---|---|
| 1 | Chladoňová Viktória | 18y + 104d | |
| 2 | Matuła Alicja | 18y + 224d | |
| 3 | Ržoncová Anna | 18y + 272d | |
| 4 | Douděrová Kateřina | 18y + 338d | |
| 5 | Cibulková Tereza | 19y + 6d | |
| 6 | Lebedová Kristýna | 19y + 47d | |
| 7 | Jurová Hana | 19y + 47d | |
| 8 | Beyerová Kateřina | 19y + 48d | |
| 9 | Slavíčková Sára | 19y + 51d | |
| 10 | Rusínová Simona | 19y + 144d | |
| 11 | Marková Adéla | 19y + 164d | |
| 12 | Dlasková Vanda | 19y + 312d | |
| 13 | Nováková Karla | 20y + 38d | |
| 14 | Jeřábková Barbora | 20y + 88d | |
| 15 | Hladíková Kateřina | 21y + 20d | |
| 16 | Vas Blanka | Team SD Worx | 23y + 177d |
| 17 | Bialek Antonina | 27y + 48d | |
| 18 | Heigl Nadja | 29y + 12d | |
| 19 | Průdková Barbora | 29y + 26d | |
| 20 | Krzystała Zuzanna | 34y + 292d | |
| 21 | Čábelická Jitka | 35y + 12d | |
| 22 | Drhová Berenika | ||
| 23 | Vágnerová Nikola | ||
| 24 | Hodinová Nicol | ||
| 25 | Kotlíková Adéla |